cd8 rabbit anti human polyclonal antibodies  (OriGene)

Journal: Breast Cancer : Targets and Therapy

Article Title: The Correlation Between CD8+ Tumor-Infiltrating Lymphocytes and the Efficacy of Neoadjuvant Therapy in Breast Cancer

doi: 10.2147/BCTT.S533799

Figure Lengend Snippet: Immunohistochemistry slides of CD8+ TILs. ( A ) Baseline CD8+ TILs under ×200 magnification. Black arrows indicate iCD8+ TILs, and red arrows indicate sCD8+ TILs. Both intratumoral and stromal CD8+ TILs are diffusely distributed, with sCD8+ TILs being more abundant than iCD8+ TILs. ( B ) Baseline CD8+ TILs under ×400 magnification, showing positive staining of the cell membrane and cytoplasm (indicated by arrows).

Article Snippet: The CD8 rabbit anti-human polyclonal antibodies and secondary antibodies were sourced from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd., and the immunohistochemistry staining kit was procured from Fuzhou Maixin Biotechnology Development Co., Ltd. Formalin-fixed, paraffin-embedded tissue sections were prepared according to standard procedures, including sectioning, deparaffinization, and rehydration.

Techniques: Immunohistochemistry, Staining, Membrane

Journal: Experimental and Therapeutic Medicine

Article Title: Overexpression of ELF1 combined with MMP9 is associated with prognosis and tumor microenvironment in gastric cancer

doi: 10.3892/etm.2024.12730

Figure Lengend Snippet: Association between ELF1 expression by tissue microarray-immunohistochemistry and that of different immune cell markers in GC.

Article Snippet: Subsequently, mouse antihuman ELF1 polyclonal antibody (dilution 1:400; cat. no. 22565-1-AP; ProteinTech Group, Inc.), MMP9 polyclonal antibody (dilution 1:300; cat. no. 10375-2-AP; ProteinTech Group, Inc.), mouse anti-human CD19 monoclonal (ready to use; cat. no. ZM-0038; ZSGB-BIO; OriGene Technologies, Inc.), mouse anti-human CD3 monoclonal (ready to use; cat. no. ZM-0417; ZSGB-BIO; OriGene Technologies, Inc.), mouse antihuman CD4 monoclonal (ready to use; cat. no. ZM-0418; ZSGB-BIO; OriGene Technologies, Inc.), rabbit anti-human CD8 monoclonal (ready to use; cat. no. ZM-0508 ZSGB-BIO; OriGene Technologies, Inc.) and mouse anti-human CD56 monoclonal (ready to use; cat. no. ZM-0057; ZSGB-BIO; OriGene Technologies, Inc.) were used for staining overnight at 4 ̊C.

Techniques: Expressing, Microarray, Significance Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Overexpression of ELF1 combined with MMP9 is associated with prognosis and tumor microenvironment in gastric cancer

doi: 10.3892/etm.2024.12730

Figure Lengend Snippet: Association of ELF1 with CD19, CD3, CD4, CD8 and CD56 detected through immunohistochemical analysis. Magnification, x100; Scale bars, 250 µm). The red arrow indicates area of high protein expression, whilst the green arrow indicates areas of low protein expression. Upper, GC with ELF1 (+); lower, GC with ELF1 (-). ELF1, E74-like Factor 1.

Article Snippet: Subsequently, mouse antihuman ELF1 polyclonal antibody (dilution 1:400; cat. no. 22565-1-AP; ProteinTech Group, Inc.), MMP9 polyclonal antibody (dilution 1:300; cat. no. 10375-2-AP; ProteinTech Group, Inc.), mouse anti-human CD19 monoclonal (ready to use; cat. no. ZM-0038; ZSGB-BIO; OriGene Technologies, Inc.), mouse anti-human CD3 monoclonal (ready to use; cat. no. ZM-0417; ZSGB-BIO; OriGene Technologies, Inc.), mouse antihuman CD4 monoclonal (ready to use; cat. no. ZM-0418; ZSGB-BIO; OriGene Technologies, Inc.), rabbit anti-human CD8 monoclonal (ready to use; cat. no. ZM-0508 ZSGB-BIO; OriGene Technologies, Inc.) and mouse anti-human CD56 monoclonal (ready to use; cat. no. ZM-0057; ZSGB-BIO; OriGene Technologies, Inc.) were used for staining overnight at 4 ̊C.

Techniques: Immunohistochemical staining, Expressing

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: Concentrations of primary antibodies.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques: Concentration Assay

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: (A) HE(x200). (B) Immunohistochemical (IHC, x400) CD8+, CD4+, FOXP3+Tregs, and PD-L1 of Tonsil (positive tissue) and breast tumor. (C) Immunofluorescence (IF, x20) Blue: DAPI; Red: CD105; Green: NG2 (The short arrow shows microvessels not covered by pericyte cells, the long arrow shows microvessels covered by pericyte cells).

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques: Immunohistochemical staining, Immunofluorescence

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: Changes of sTILs, PD-L1, MVD, and MPI in non-pCR group and pCR group after NAC.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: Univariate and multivariate analysis of pCR after NAC.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: Relationship between the baseline ratios of populations and pCR.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: The relationship between the changes of sTILs, PD-L1, MVD, MPI, and DFS before and after NAC. (A) Survival analysis between pCR group and Non-pCR group. (B) Survival analysis of sTILs change. (C) Survival analysis of CD8+T change. (D) Survival analysis of CD4+T change. (E) Survival analysis of FOXP3+ Tregs change. (F) Survival analysis of PD-L1 change. (G) Survival analysis of MVD change. (H) Survival analysis of MPI change.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: The relationship between DFS and sTILs, PD-L1, MVD, and MPI in Metastatic lymph node (LN). (A) Survival analysis of LN sTILs. (B) Survival analysis of LN CD8+ T cells. (C) Survival analysis of LN CD4+ T cells. (D) Survival analysis of LN FOXP3+ Tregs. (E) Survival analysis of LN PD-L1. (F) Survival analysis of LN MVD. (G) Survival analysis of LN MPI.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Association between the numbers of CD4- and CD8-positive lymphocytes in hepatocellular carcinoma tumor parenchymal tissues, and patient clinicopathological characteristics.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Biomarker Discovery

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Association between the numbers of CD4- and CD8-positive lymphocytes in hepatocellular carcinoma tumor stromal tissues, and patient clinicopathological characteristics.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Biomarker Discovery

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Immunohistochemical analysis of T-cell expression in the TP and TS of hepatocellular carcinoma samples (original magnification, ×400). (A) CD4 + and (B) CD8 + T-cell expression in TP and TS. (C) Morphometrical analysis was performed. Data are presented as the mean ± standard error of the mean. ** P<0.01, TP vs. TS in CD4 and CD8; †† P<0.01, CD4 vs. CD8 in TP; † P<0.05, CD4 vs. CD8 in TS. Statistical analyses were performed using the Mann-Whitney U test. TP, tumor parenchyma; TS, tumor stroma; h.p.f., high-power field.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Immunohistochemical staining, Expressing, MANN-WHITNEY

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Immunohistochemical analysis of CD8 + T-cell expression in the TP and TS of hepatocellular carcinoma samples (original magnification, ×400). CD8 + T-cell expression in patients with tumor diameters (A) ≤5 cm and (B) >5 cm. (C) Morphometrical analysis was performed. Data are presented as the mean ± standard error of the mean. * P<0.05, diameter ≤5 cm group vs. diameter >5 cm group in TP and TS. TP, tumor parenchyma; TS, tumor stroma; h.p.f., high-power field.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Immunohistochemical staining, Expressing

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: CD8 expression in hepatocellular carcinoma (TP) and paired peritumor liver tissues (PTP). Morphometrical analysis was performed for semi-quantitative evaluation of the immunohistochemical findings. Data are presented as the mean ± standard error of the mean. Statistical analysis was performed using the nonparametric Mann-Whitney U test. ** P<0.01, PTP vs. TP in CH and C background groups. NCH, non-chronic hepatitis; CH, chronic hepatitis; PC, pre-cirrhotic stage; C, cirrhosis; PTP, peritumor parenchyma; TP, tumor parenchyma; h.p.f., high-power field.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Expressing, Immunohistochemical staining, MANN-WHITNEY

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Correlation between CD4 and CD8 expression in (A) TP and (B) TS of HCC samples. Statistical analyses were performed using Spearman’s correlation coefficient by rank test. P<0.001. TP, tumor parenchyma; TS, tumor stroma; HCC, hepatocellular carcinoma; h.p.f., high-power field.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Expressing

Journal: The Journal of Experimental Medicine

Article Title: Activated monocytes in peritumoral stroma of hepatocellular carcinoma foster immune privilege and disease progression through PD-L1

doi: 10.1084/jem.20082173

Figure Lengend Snippet: Tumor-derived monocytes induced T cell suppression via PD-L1. (A) Physical contact between PD-L1 + cells (red) and CD8 + cytotoxic T cells (green) in HCC peritumoral stroma. One out of five representative micrographs is shown in A. Bar, 20 µm. (B and C) Increased expression of PD-1 protein on the surface of tumor-infiltrating T cells from HCC patients. The samples used in B and C were fresh blood from healthy individuals and HCC patients, and paired nontumor and tumor tissues from HCC patients ( n = 7 for each). The data shown in B are representative dot plots of seven patients from six independent experiments. The horizontal bars in C represent median values. (D) HCC-derived monocytes induced anergy of tumor T cells with reduced IFN-γ production. Purified tumor T cells were left untreated (1), or were incubated for 20 h with autologous tumor monocytes supplemented with 25 µg/ml of autologous tumor mass lysate in the absence (2) or presence of 5 µg/ml of control (3) or anti–PD-L1 antibody (4). Thereafter, production of IFN-γ was determined by ELISPOT. Three out of six representative patient samples are shown in D.

Article Snippet: For immunofluorescence analysis, tissues were stained with polyclonal rabbit anti–human CD8 and mouse anti–human PD-L1, or rabbit anti–human CD68 (Santa Cruz Biotechnology, Inc.) and mouse anti–human PD-L1, followed by Alexa Fluor 488– or 568–conjugated goat anti–mouse IgG and Alexa Fluor 568– or 488–conjugated goat anti–rabbit IgG (Invitrogen).

Techniques: Derivative Assay, Expressing, Purification, Incubation, Control, Enzyme-linked Immunospot

Journal: The Journal of Experimental Medicine

Article Title: Activated monocytes in peritumoral stroma of hepatocellular carcinoma foster immune privilege and disease progression through PD-L1

doi: 10.1084/jem.20082173

Figure Lengend Snippet: TSN-exposed monocytes induced T cell suppression via PD-L1. Autologous monocytes (MO) or TSN-treated monocytes (PD-L1 + MO) were pretreated with 10 µg/ml mitomycin C for 30 min and were then washed and incubated with tumor-specific T cells (1:10) in the presence or absence of 5 µg/ml anti–PD-L1 or control antibody, as described in Materials and methods. The expression of CD25 on (A) T cells, (B) perforin in CD8 + T cells, and (C) intracellular staining of IFN-γ were determined by FACS, and (D) the secretion of cytokines and proliferation of T cells were determined by ELISA and BrdU assay, respectively. The results shown are representative of at least four separate experiments and are expressed as means ± SEM. Significant differences compared with normal monocytes are indicated (*, P < 0.05; and **, P < 0.01).

Article Snippet: For immunofluorescence analysis, tissues were stained with polyclonal rabbit anti–human CD8 and mouse anti–human PD-L1, or rabbit anti–human CD68 (Santa Cruz Biotechnology, Inc.) and mouse anti–human PD-L1, followed by Alexa Fluor 488– or 568–conjugated goat anti–mouse IgG and Alexa Fluor 568– or 488–conjugated goat anti–rabbit IgG (Invitrogen).

Techniques: Incubation, Control, Expressing, Staining, Enzyme-linked Immunosorbent Assay, BrdU Staining